Abstract

Research Article

Cytokine Modulatory Effects of Sesamum Indicum Seeds Oil Ameliorate Mice with Experimental Autoimmune Encephalomyelitis

Mohammad Reza Javan*, Mohammad Reza Zamani, Saeed Aslani, Ghader Dargahi Abbasabad, Masoud Beirami Khalaj and Hamed Serati-Nouri

Published: 30 October, 2017 | Volume 1 - Issue 1 | Pages: 086-093

Background: Multiple sclerosis (MS) is an autoimmune disorder of the central nerve system (CNS), which affects the brain and spinal cord. Experimental autoimmune encephalomyelitis (EAE) is the most commonly applied experimental model for studying the MS. The aim of this study was to determine the effects of Sesamum indicum seeds oil on Experimental Autoimmune Encephalomyelitis (EAE) in mice.

Methods: Sesame oil was administrated intraperitoneally three days before immunization. IFN-γ, IL-10, IL-17 and TGF-β levels and mRNA expression in supernatant of and within cultured mononuclear cells were assessed.

Results: According to our results, sesame oil treated mice demonstrated significant disease severity reduction (P=0.01 and 0.001, respectively). Treated EAE mice also represented statistically significant delay in the onset of symptoms in comparison with control group. The average IFN-γ levels and mRNA of sesame oil treated EAE mice were less than untreated EAE group. IL-10 and TGF-β levels and mRNA did not differ significantly in sesame oil treated EAE mice in comparison to untreated EAE group. IL-17 levels and mRNA were also found to be decreased significantly in treated mice in comparison to untreated mice.

Conclusions: Even thoughTH1 and TH17 cells through secretion of IFN-γ and IL-17, respectively, are involved in the pathogenesis of multiple sclerosis and EAE, but IL-10 has been shown to exhibit suppressive effects on these disorders. It can be concluded that sesame oil is able to induce TH2 and TH17-related immune responses and suppress TH1 type in EAE

Read Full Article HTML DOI: 10.29328/journal.aaai.1001008 Cite this Article Read Full Article PDF

Keywords:

EAE; MS; Gene expression; Cytokine; Sesamum indicum seeds oil

References

  1. Ransohoff RM. Animal models of multiple sclerosis: the good, the bad and the bottom line. Nat Neurosci. 2012; 15: 1074-1077. Ref.: https://goo.gl/MVZUjC
  2. Rengarajan J, Szabo SJ, Glimcher LH. Transcriptional regulation of Th1/Th2 polarization. Immunol Today. 2000; 21: 479-483. Ref.: https://goo.gl/oTL9LX
  3. de Andrés C, Rodríguez‐Sáinz MC, Muñoz‐Fernández MA, López‐Lazareno N, Rodríguez‐Mahou M, et al. Short‐term sequential analysis of sex hormones and helper T cells type 1 (Th1) and helper T cells type 2 (Th2) cytokines during and after multiple sclerosis relapse. Eur Cytokine Netw. 2004; 15: 197-202. Ref.: https://goo.gl/LDHzZf
  4. Sakaguchi S. Editor Regulatory T cells. Springer seminars in immunopathology. 2006.
  5. Li MO, Wan YY, Sanjabi S, Robertson AK, Flavell RA. Transforming growth factor-β regulation of immune responses. Annu Rev Immunol. 2006; 24: 99-146. Ref.: https://goo.gl/xg5nWv
  6. Faria AMC, Maron R, Ficker SM, Slavin AJ, Spahn T, et al. Oral tolerance induced by continuous feeding: enhanced up-regulation of transforming growth factor-β/interleukin-10 and suppression of experimental autoimmune encephalomyelitis. J Autoimmun. 2003; 20: 135-145. Ref.: https://goo.gl/iq1wNw
  7. John GR, Shankar SL, Shafit-Zagardo B, Massimi A, Lee SC, et al. Multiple sclerosis: re-expression of a developmental pathway that restricts oligodendrocyte maturation. Nat Med. 2002; 8: 1115-1121. Ref.: https://goo.gl/DT6Gvj
  8. Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, et al. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. J Exp Med. 2001; 194: 519-528. Ref.: https://goo.gl/NL8yFo
  9. Hsu DZ, Su SB, Chien SP, Chiang PJ, Li YH, et al. Effect of sesame oil on oxidative-stress-associated renal injury in endotoxemic rats: involvement of nitric oxide and proinflammatory cytokines. Shock. 2005; 24: 276-280. Ref.: https://goo.gl/4wurMJ
  10. Gangur V, Kelly C, Navuluri L. Sesame allergy: a growing food allergy of global proportions? Ann Allergy Asthma Immunol. 2005; 95: 4-11. Ref.: https://goo.gl/iCDzuC
  11. Fillatreau S, Sweenie CH, McGeachy MJ, Gray D, Anderton SM. B cells regulate autoimmunity by provision of IL-10. Nat Immun. 2002; 3: 944-950. Ref.: https://goo.gl/nPkMsk
  12. Hsu DZ, Liu MY. Sesame oil protects against lipopolysaccharide-stimulated oxidative stress in rats. Crit Care Med. 2004; 32: 227-231. Ref.: https://goo.gl/SJvknG
  13. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008; 3: 1101-1108. Ref.: https://goo.gl/qKczfW
  14. Lassmann H, van Horssen J, Mahad D. Progressive multiple sclerosis: pathology and pathogenesis. Nat Rev Neurol. 2012; 8: 647-656. Ref.: https://goo.gl/978jrj
  15. Jiang HR, Milovanović M, Allan D, Niedbala W, Besnard AG, et al. IL‐33 attenuates EAE by suppressing IL‐17 and IFN‐γ production and inducing alternatively activated macrophages. Eur J Immunol. 2012; 42: 1804-1814. Ref.: https://goo.gl/agPZNY
  16. Tyler A, Mendoza J, Ortega S, Firan M, Karandikar N. CD8+ T-cells are required for the action of glatiramer acetate therapy for autoimmune demyelinating disease. J Immunol. 2011; 186: 164.119. Ref.: https://goo.gl/e6og8R
  17. Guinness NM, Dungan LS, Lynch MA, Mills KH. Interferon-gamma-producing natural killer cells are pathogenic in experimental autoimmune encephalomyelitis by promoting M1 macrophage activation and VLA-4 expression on CD4+ T cells. J Neuroimmunol. 2014; 275: 119-120. Ref.: https://goo.gl/fud1vC
  18. Lin W, Lin Y. Interferon-gamma inhibits central nervous system myelination through both STAT1-dependent and STAT1-independent pathways. J Neurosci Res. 2010; 88: 2569-2577. Ref.: https://goo.gl/jRvzdr
  19. Jacklin A, Ratledge C, Welham K, Bilko D, Newton CJ. The sesame seed oil constituent, sesamol, induces growth arrest and apoptosis of cancer and cardiovascular cells. Ann N Y Acad Sci. 2003; 1010: 374-380. Ref.: https://goo.gl/cieHrT
  20. Miyahara Y, Hibasami H, Katsuzaki H, Imai K, Komiya T. Sesamolin from sesame seed inhibits proliferation by inducing apoptosis in human lymphoid leukemia Molt 4B cells. Int J Mol Med. 2001; 7: 369-371. Ref.: https://goo.gl/dR2atC
  21. Salem ML. Systemic treatment with n-6 polyunsaturated fatty acids attenuates EL4 thymoma growth and metastasis through enhancing specific and non-specific anti-tumor cytolytic activities and production of TH1 cytokines. Int Immunopharmacol. 2005; 5: 947-960. Ref.: https://goo.gl/M5H3vN
  22. Komiyama Y, Nakae S, Matsuki T, Nambu A, Ishigame H, et al. IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis. J Immunol. 2006; 177: 566-573. Ref.: https://goo.gl/1sx7ME
  23. Langrish CL, Chen Y, Blumenschein WM, Mattson J, Basham B, et al. IL-23 drives a pathogenic T cell population that induces autoimmune inflammation. J Exp Med. 2005; 201: 233-240. Ref.: https://goo.gl/ga4c2X
  24. Skapenko A, Niedobitek GU, Kalden JR, Lipsky PE, Schulze-Koops H. Generation and regulation of human Th1-biased immune responses in vivo: a critical role for IL-4 and IL-10. J Immunol. 2004; 172: 6427-6434. Ref.: https://goo.gl/2dYa8T
  25. O’Neill EJ, Day MJ, Wraith DC. IL-10 is essential for disease protection following intranasal peptide administration in the C57BL/6 model of EAE. J Neuroimmunol. 2006; 178: 1-8. Ref.: https://goo.gl/C79y9f
  26. Santos L, Al-Sabbagh A, Londono A, Weiner HL. Oral tolerance to myelin basic protein induces regulatory TGF-β-secreting T cells in Peyer's patches of SJL mice. Cell Immunol. 1994; 157: 439-447. Ref.: https://goo.gl/MhkYHF
  27. Johns L, Flanders K, Ranges G, Sriram S. Successful treatment of experimental allergic encephalomyelitis with transforming growth factor-beta 1. J Immunol. 1991; 147: 1792-1796. Ref.: https://goo.gl/DwZTmo

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